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  • BSI
    BS EN ISO 9308-2:2014 Water quality. Enumeration of Escherichia coli and coliform bacteria - Most probable number method
    Edition: 2014
    $497.46
    / user per year

Description of BS EN ISO 9308-2:2014 2014

This part of ISO 9308 specifies a method for the enumeration of E. coli and coliform bacteria in water. The method is based on the growth of target organisms in a liquid medium and calculation of the “Most Probable Number” (MPN) of organisms by reference to MPN tables. This method can be applied to all types of water, including those containing an appreciable amount of suspended matter and high background counts of heterotrophic bacteria. However, it must not be used for the enumeration of coliform bacteria in marine water. When using for the enumeration of E. coli in marine waters, a 1→10 dilution in sterile water is typically required, although the method has been shown to work well with some marine waters that have a lower than normal concentration of salts. In the absence of data to support the use of the method without dilution, a 1→10 dilution is used.

This method relies upon the detection of E. coli based upon expression of the enzyme β-D-glucuronidase and consequently does not detect many of the enterohaemorhagic strains of E. coli, which do not typically express this enzyme. Additionally, there are a small number of other E. coli strains that do not express β-D-glucuronidase.

The choice of tests used in the detection and confirmation of the coliform group of bacteria, including E. coli, can be regarded as part of a continuous sequence. The extent of confirmation with a particular sample depends partly on the nature of the water and partly on the reasons for the examination. The test described in this part of ISO 9308 provides a confirmed result with no requirement for further confirmation of positive wells.

NOTE While this method describes the use of an enumeration device that is commercially available, the medium described here can also be used in a standard MPN format.



Standard NumberBS EN ISO 9308-2:2014
TitleWater quality. Enumeration of Escherichia coli and coliform bacteria - Most probable number method
StatusUnder Review
Publication Date2014-06-30
Identical National Standard OfEN ISO 9308-2:2014, ISO 9308-2:2012
CorrectsBS ISO 9308-2:2012
DescriptorsMicrobiological analysis, Water, Water testing, Bacteria count methods, Escherichia coli, Count methods (microbiology), Coliform bacteria, Microorganisms, Bacteria, Counting
ICS07.100.20 Microbiology of water
CommitteeEH/3/4
ISBN978 0 580 84023 4
PublisherBSI
Pages56

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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