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  • BSI
    BS EN ISO 9167:2019 - TC Tracked Changes. Rapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography
    Edition: 2020
    $545.49
    / user per year

Description of BS EN ISO 9167:2019 - TC 2020

This document specifies a method for the determination of the individual glucosinolates content in rapeseeds and rapeseed meals using high-performance liquid chromatography with gradient elution.

This method was tested on rapeseeds and rapeseed meals (Brassica rapa, Brassica napus and Brassica juncea) but is applicable to other plant materials, on the condition that the occurring glucosinolates previously identified are described in this document. On the contrary, the quantitative analysis of the concerned glucosinolate(s) is not carried out.

NOTE

This method does not determine glucosinolates that are substituted on the glucose molecule, but these compounds are of little importance in commercial rapeseed and rapeseed meal.

Annex A presents the results of the interlaboratory trials for the gradient elution HPLC method. Annex B presents how to check the titre of the prepared internal standard solution. Annex C presents how to prepare and test the purified sulfatase solution and how to check the desulphation step on the ion exchange column. Annex D presents the HPLC and column performance criteria qualification.

The analysis of glucosinolates content in rapeseed can also be done using an isocratic elution mode. This requires some modifications of the method (internal, standard, HPLC column and HPLC buffers), as described in Annex E.



Standard NumberBS EN ISO 9167:2019 - TC
TitleTracked Changes. Rapeseed and rapeseed meals. Determination of glucosinolates content. Method using high-performance liquid chromatography
StatusDefinitive
Publication Date2020-02-24
DescriptorsDetermination of content, Liquid chromatography, Reproducibility, Test equipment, Plant products, Rape seed oil, Measurement characteristics, Control samples, Column chromatography, Extraction methods of analysis, Quantitative analysis, Chemical analysis and testing, Specimen preparation, Chromatography, Cruciferae, Testing conditions, Vegetable oils, Test specimens
ICS67.200.20 Oilseeds
CommitteeAW/307
ISBN978-0-539-07739-1
PublisherBSI
Pages0

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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