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  • BSI
    BS EN ISO 20418-1:2018 Textiles. Qualitative and quantitative proteomic analysis of some animal hair fibres - Peptide detection using LC-ESI-MS with protein reduction
    Edition: 2018
    $316.70
    / user per year

Description of BS EN ISO 20418-1:2018 2018

This document specifies a qualitative and quantitative testing method to determine the content of wool, cashmere, yak fibres and their blends in textiles by microscope preliminary screening, protein extraction, enzymatic digestion and specific peptides detection using a liquid chromatography-mass spectrometer equipped with electrospray ionization source (LCI-ESI-MC).

This method can be applied to relevant textile products at each process stage (i.e. from raw material to garment) with a homogeneous distribution of the components. It can be applied to different types of textile materials (e.g. staples, tops, yarns and fabrics) that contain wool, cashmere or yak fibres and their blends. The method is based on a preliminary identification of all fibres in the blend on the basis of their morphology, by light microscopy. The proteins are then extracted by a thiourea/urea/dithiothreitol (DTT) solution. An enzymatic digestion by trypsin of the protein extracted from the fibres is carried out. Analysis of the specific markers is performed by LC-MS and the percent composition is calculated.

This method is applicable to samples containing other kinds of fibres than wool, cashmere and yak, by combining its results with the results obtained using the ISO 1833 series and/or the ISO 17751 series.

This document does not apply if fibres of the same animal species are present (e.g. blends of cashmere and mohair); in this case, the quantitative analysis can be performed using microscopic analysis (e.g. ISO 17751 series).



Standard NumberBS EN ISO 20418-1:2018
TitleTextiles. Qualitative and quantitative proteomic analysis of some animal hair fibres - Peptide detection using LC-ESI-MS with protein reduction
StatusConfirmed
Publication Date2018-06-05
Identical National Standard OfISO 20418-1:2018, EN ISO 20418-1:2018
DescriptorsTextile products, Textiles, Wool, Quantitative analysis, Qualitative analysis
ICS59.060.01 Textile fibres in general
CommitteeTCI/100
ISBN978 0 580 89702 3
PublisherBSI
Pages20

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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