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  • BSI
    BS EN 15634-2:2019 Foodstuffs. Detection of food allergens by molecular biological methods - Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR
    Edition: 2019
    $411.69
    / user per year

Description of BS EN 15634-2:2019 2019

This document specifies a method for the detection of celery (Apium graveolens) in emulsion-type sausages (e.g. Frankfurter, Wiener).

Real-time PCR (polymerase chain reaction) detection of celery is based on an 101 bp (base pair) sequence from the gene of the mannitol dehydrogenase (GenBank Acc. No. AF067082 1) of celery (Apium graveolens).

The method has been validated on emulsion-type sausages (Bavarian “Leberkäse”) spiked with celery. For this purpose meat batter containing mass fractions of 50 % pork meat, 25 % pork fat, 23 % crushed ice and 1,8 % of a mixture of sodium chloride, nitrite, nitrate, phosphates and ascorbates was prepared according to a standard procedure for emulsion-type sausage. The meat batter was spiked with either ground celery seeds or celery root powder to 1000 mg/kg. Lower spiking levels were obtained by diluting with celery-free meat batter. The batter was stuffed into casings and heated at 65 °C for 60 min [1].

This document is intended to be used in addition to EN 15842 and EN 15634?1.



Standard NumberBS EN 15634-2:2019
TitleFoodstuffs. Detection of food allergens by molecular biological methods - Celery (Apium graveolens). Detection of a specific DNA sequence in cooked sausages by real-time PCR
StatusDefinitive
Publication Date2019-10-11
ReplacesPD CEN/TS 15634-2:2012
Identical National Standard OfEN 15634-2
DescriptorsQualitative analysis, Allergies, Food testing, Agricultural products, Biochemical methods of analysis
ICS67.120.10 Meat and meat products, 07.100.30 Food microbiology
CommitteeAW/275
ISBN978 0 580 99646 7
PublisherBSI
Pages24

About BSI

BSI Group, also known as the British Standards Institution is the national standards body of the United Kingdom. BSI produces technical standards on a wide range of products and services and also supplies certification and standards-related services to businesses.
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