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eLibrary > BS EN 17122:2019 Chemical disinfectants and antiseptics. Quantitative non- porous surface test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements. Phase2, step2 >

BS EN 17122:2019 Chemical disinfectants and antiseptics. Quantitative non- porous surface test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements. Phase2, step2, 2019

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BS EN 17122:2019 Chemical disinfectants and antiseptics. Quantitative non- porous surface test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements. Phase2, step2, 2019

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1 Scope
2 Normative references
3 Terms and definitions
4 Requirements for virucidal activity on surfaces
5 Test method [Go to Page]
5.1 Principle [Go to Page]
5.1.1 Outline
5.1.2 Test Organisms
5.1.3 Variations
5.2 Materials and reagents, including cell cultures [Go to Page]
5.2.1 Test organisms
5.2.2 Culture media, reagents and cell cultures [Go to Page]
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Phosphate buffered saline (PBS)
5.2.2.4 Neutral Red (1:1000 solution)
5.2.2.5 Foetal calf serum (FCS)
5.2.2.6 Trichloroacetic acid (10 % solution) (TCA)
5.2.2.7 Hard water for dilution of products
5.2.2.8 Interfering substance
5.2.2.9 Medium for cell cultures
5.2.2.10 Cell cultures
5.3 Apparatus and glassware [Go to Page]
5.3.1 General
5.3.2 Usual microbiological laboratory equipment
5.3.3 Test surface
5.4 Preparation of test organism suspensions and product test solutions [Go to Page]
5.4.1 Test organism suspensions (test virus suspension)
5.4.2 Product test solution
5.5 Procedure for assessing the virucidal activity of the product [Go to Page]
5.5.1 Experimental conditions
5.5.2 Test procedure
5.5.3 Cytotoxicity caused by product solutions [Go to Page]
5.5.3.1 Cytotoxic effect
5.5.3.2 Interference control – control of cell susceptibility
5.5.3.3 Elimination of cytotoxicity
5.5.4 Control of efficiency for suppression of disinfectant virucidal activity [Go to Page]
5.5.4.1 Dilution in ice-cold medium
5.5.4.2 Filtration technique
5.5.5 Titration of the virus control
5.6 Experimental data and calculation [Go to Page]
5.6.1 Protocol of the results
5.6.2 Calculation of infectivity titre (TCID50 – PFU) [Go to Page]
5.6.2.1 General
5.6.2.2 Calculation of TCID50
5.6.2.3 Calculation of PFU
5.7 Verification of the methodology
5.8 Expression of results [Go to Page]
5.8.1 General
5.8.2 Calculation of the virucidal activity of products
5.9 Test report
Annex A (informative)Referenced strains of national collections
Annex B (informative) [Go to Page]
B.1 Molecular sieving with SephadexTM LH 20 [Go to Page]
B.1.1 Principle
B.1.2 SephadexTM suspension
B.1.3 Procedure
B.2 Molecular sieving using MicroSpinTM S 400 HR
Annex C (informative)Calculation of virus infectivity titre [Go to Page]
C.1 Quantal tests - Example of TCID50 determination by the Spaerman-Kärber method
C.2 Plaque test
C.3 Biometrical evaluation of experimental approaches and assessment of the disinfecting effect on the virus (reduction [R]): [Go to Page]
C.3.1 General
C.3.2 Calculating the virus titre with 95 % confidence interval - Example
C.3.3 Calculating the reduction and its 95 % confidence interval
C.3.4 Calculating the average reduction (R(mi)) and its 95 % confidence interval
C.3.5 Practical example
Annex D (informative)Example of a typical test report
Annex E (informative)Full virucidal activity and activity against enveloped viruses only [Go to Page]

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BS EN 17122:2019 Chemical disinfectants and antiseptics. Quantitative non- porous surface test for the evaluation of virucidal activity of chemical disinfectants and antiseptics used in the veterinary area. Test method and requirements. Phase2, step2, 2019

To continue with accurate pricing,
please select your company type below: