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eLibrary > BS EN 1276:2019 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas. Test method and requirem >

BS EN 1276:2019 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas. Test method and requirem, 2019

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BS EN 1276:2019 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas. Test method and requirem, 2019

National foreword
1 Scope
2 Normative references
3 Terms and definitions
4 Requirements
5 Test method [Go to Page]
5.1 Principle
5.2 Materials and reagents [Go to Page]
5.2.1 Test organisms
5.2.2 Culture media and reagents [Go to Page]
5.2.2.1 General
5.2.2.2 Water
5.2.2.3 Tryptone Soya Agar (TSA)
5.2.2.4 Diluent
5.2.2.5 Neutralizer
5.2.2.6 Rinsing liquid (for membrane filtration)
5.2.2.7 Hard water for dilution of products
5.2.2.8 Interfering substance
5.3 Apparatus and glassware [Go to Page]
5.3.1 General
5.3.2 Usual microbiological laboratory equipment) and, in particular, the following
5.4 Preparation of test organism suspensions and product test solutions [Go to Page]
5.4.1 Test organism suspensions (test and validation suspension) [Go to Page]
5.4.1.1 General
5.4.1.2 Preservation and stock cultures of test organisms
5.4.1.3 Working culture of test organisms
5.4.1.4 Test suspension (“N”)
5.4.1.5 Validation suspension (“Nv”)
5.4.1.6 Incubation and counting of the test and the validation suspensions
5.4.2 Product test solutions
5.5 Procedure for assessing the bactericidal activity of the product [Go to Page]
5.5.1 General [Go to Page]
5.5.1.1 Experimental conditions
5.5.1.2 Choice of test method (dilution-neutralization or membrane filtration)
5.5.1.3 General instructions for validation and control procedures
5.5.1.4 Equilibration of temperature
5.5.1.5 Precautions for manipulation of test organisms
5.5.2 Dilution-neutralization method) [Go to Page]
5.5.2.1 General
5.5.2.2 Test “Na” – determination of bactericidal concentrations
5.5.2.3 Experimental conditions control “A” – validation of the selected experimental conditions and/or verification of the absence of any lethal effect in the test conditions
5.5.2.4 Neutralizer control “B” – verification of the absence of toxicity of the neutralizer
5.5.2.5 Method validation “C” – dilution-neutralization validation
5.5.2.6 Incubation and counting of the test mixture and the control and validation mixtures
5.5.3 Membrane filtration method) [Go to Page]
5.5.3.1 General
5.5.3.2 Test “Na” – determination of the bactericidal concentrations
5.5.3.3 Experimental conditions control “A” – validation of the selected experimental conditions and/or verification of the absence of any lethal effect in the test conditions
5.5.3.4 Filtration control “B” – validation of the filtration procedure
5.5.3.5 Method validation “C” – validation of the membrane filtration method or counting of the bacteria on the membranes which have previously been in contact with the mixture of product and interfering substance
5.5.3.6 Incubation and counting of test mixture and the control and the validation mixtures
5.6 Experimental data and calculation [Go to Page]
5.6.1 Explanation of terms and abbreviations [Go to Page]
5.6.1.1 Overview of the different suspensions and test mixtures
5.6.1.2 Vc values
5.6.2 Calculation [Go to Page]
5.6.2.1 General
5.6.2.2 Determination of Vc values
5.6.2.3 Calculation of N and N0
5.6.2.4 Calculation of Na
5.6.2.5 Calculation of Nv and Nvo
5.6.2.6 Calculation of A, B and C
5.7 Verification of methodology [Go to Page]
5.7.1 General
5.7.2 Control of weighted mean counts
5.7.3 Basic limits
5.8 Expression of results and precision [Go to Page]
5.8.1 Reduction
5.8.2 Control of active and non-active product test solution (5.4.2)
5.8.3 Limiting test organism and bactericidal concentration
5.8.4 Precision, replicates
5.9 Interpretation of results - conclusion [Go to Page]
5.9.1 General
5.9.2 Bactericidal activity for general purposes
5.9.3 Bactericidal activity for hand hygiene
5.10 Test report
Annex A (informative)Referenced strains in national collections
Annex B (informative)Neutralizers and rinsing liquids
Annex C (informative)Graphical representations of dilution neutralization method and membrane filtration method [Go to Page]
C.1 Dilution-neutralization method
C.2 Membrane filtration method
Annex D (informative)Example of a typical test report
Annex E (informative)Precision of the test result [Go to Page]

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BS EN 1276:2019 Chemical disinfectants and antiseptics. Quantitative suspension test for the evaluation of bactericidal activity of chemical disinfectants and antiseptics used in food, industrial, domestic and institutional areas. Test method and requirem, 2019

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