FORMAT
BOOKS
PACKAGES
EDITION
PUBLISHER
CONTENT TYPE
Act
Admin Code
Announcements
Bill
Book
CADD File
CAN
CEU
Charter
Checklist
City Code
Code
Commentary
Comprehensive Plan
Conference Paper
County Code
Course
DHS Documents
Document
Errata
Executive Regulation
Federal Guideline
Firm Content
Guideline
Handbook
Interpretation
Journal
Land Use and Development
Law
Legislative Rule
Local Amendment
Local Code
Local Document
Local Regulation
Local Standards
Manual
Model Code
Model Standard
Notice
Ordinance
Other
Paperback
PASS
Periodicals
PIN
Plan
Policy
Product
Program
Provisions
Requirements
Revisions
Rules & Regulations
Standards
State Amendment
State Code
State Manual
State Plan
State Standards
Statute
Study Guide
Supplement
Technical Bulletin
All
|
Description of ASTM-D4454 2009ASTM D4454 - 85(2009)Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by MicroscopyActive Standard ASTM D4454 | Developed by Subcommittee: D19.24 Book of Standards Volume: 11.02 ASTM D4454Significance and Use Measurement of bacterial densities is generally the first step in establishing a relationship between bacteria and other biochemical processes. It is known that the classical plate count procedure underestimates bacterial densities while the epifluorescence direct microscopic procedure more accurately depicts the total numbers of nonviable or dormant and viable cells in a water sample. The acridine-orange INT-formazan reduction technique provides information on the total concentrations of bacteria as well as that proportion which are actively respiring and thus involved in degradative processes. The acridine-orange INT-formazan reduction technique is both quantitative and precise. This procedure is ideal for enumerating both pelagic and epibenthic bacteria in all fresh water and marine environments. The process can be employed in survey studies to characterize the bacteriological densities and activities of environmental waters. 1. Scope 1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters. 1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears. 1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications. 1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends. 1.5 Approximately 10 4 cells/mL are required for detection by this test method. 1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 ? m. 2 1.7 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard. 1.8 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
ASTM Standards D1129 Terminology Relating to Water D1193 Specification for Reagent Water Keywords Water-formed deposits; Bacteria/bacterial control--water; Epifluorescence microscopy; Fluorescence--water analysis; Microscopic examination--water; Pathogens--water; ICS Code ICS Number Code 07.100.20 (Microbiology of water) DOI: 10.1520/D4454-85R09 ASTM International is a member of CrossRef. ASTM D4454This book also exists in the following packages...Subscription InformationMADCAD.com ASTM Standards subscriptions are annual and access is unlimited concurrency based (number of people that can access the subscription at any given time) from single office location. For pricing on multiple office location ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
Some features of MADCAD.com ASTM Standards Subscriptions are: - Immediate Access: As soon as the transaction is completed, your ASTM Standards Subscription will be ready for access.
For any further information on MADCAD.com ASTM Standards Subscriptions, please contact us at info@madcad.com or +1 800.798.9296.
About ASTMASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide. |
GROUPS
|