ASTM D4454-85(2002)
Historical Standard: ASTM D4454-85(2002) Standard Test Method for Simultaneous Enumeration of Total and Respiring Bacteria in Aquatic Systems by Microscopy
SUPERSEDED (see Active link, below)
ASTM D4454
1. Scope
1.1 This test method covers the detection and enumeration of aquatic bacteria by the use of an acridine-orange epifluorescence direct-microscopic counting procedure. This test method is applicable to environmental waters and potable waters.
1.2 Certain types of debris and other microorganisms may fluoresce in acridine-orange stained smears.
1.3 The procedure described requires a trained microbiologist or technician who is capable of distinguishing bacteria from other fluorescing bodies on the basis of morphology when viewed at higher magnifications.
1.4 Use of bright light permits differentiation of single bacteria where reduced formazan is deposited at the polar ends.
1.5 Approximately 10 4 cells/mL are required for detection by this test method.
1.6 Minimal cell size which allows the detection of formazan deposits is represented by bacteria of 0.4 m. 2
1.7 This standard does not purport to address the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.
2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.
ASTM Standards
D1129 Terminology Relating to Water
D1193 Specification for Reagent Water
Keywords
Water-formed deposits; Bacteria/bacterial control--water; Epifluorescence microscopy; Fluorescence--water analysis; Microscopic examination--water; Pathogens--water;
ICS Code
ICS Number Code 07.100.20 (Microbiology of water)
DOI: 10.1520/D4454-85R02
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