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  • ASTM
    F3088-14 Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions
    Edition: 2014
    $93.60
    Unlimited Users per year

Description of ASTM-F3088 2014

ASTM F3088-14

Historical Standard: Standard Test Method for Use of a Centrifugation Method to Quantify/Study Cell-Material Adhesive Interactions




ASTM F3088

Scope

1.1 This test method covers a centrifugation cell adhesion assay that can be used to detect changes in adhesive characteristics of cells with passage or treatments. This approach measures the force required to detach cells from a substrate. Adhesion, among many variables, may vary due to changes in the phenotype of the cells.

1.2 This test method does not cover methods to verify the uniformity of coating of surfaces, nor does it cover methods for characterizing surfaces.

1.3 The cells may include adult, progenitor or stem cells from any species. The types of cells may include chondrocytes, fibroblasts, osteoblast, islet cells, or other relevant adherent cell types.

1.4 This test method does not cover methods for isolating or harvesting of cells. This test method does not cover test methods to quantitate changes in gene expression, or changes in biomarker type or concentration, as identified by immunostaining. Nor does this test method cover quantitative image analysis techniques.

1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


Keywords

cell adhesion assay; centrifugation; chondrocytes; monolayer culture; serial passage; tissue culture-treated polystyrene


ICS Code

ICS Number Code 11.100.99 (Other standards related to laboratory medicine)


DOI: 10.1520/F3088-14

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About ASTM

ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.
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