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  • ASTM
    E2799-11 Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay
    Edition: 2011
    $93.60
    Unlimited Users per year

Description of ASTM-E2799 2011

ASTM E2799-11

Historical Standard: ASTM E2799-11 Standard Test Method for Testing Disinfectant Efficacy against Pseudomonas aeruginosa Biofilm using the MBEC Assay

SUPERSEDED (see Active link, below)




ASTM E2799

1. Scope

1.1 This test method specifies the operational parameters required to grow and treat a Pseudomonas aeruginosa biofilm in a high throughput screening assay known as the MBEC (trademarked) (Minimum Biofilm Eradication Concentration) Physiology and Genetics Assay. The assay device consists of a plastic lid with ninety-six (96) pegs and a corresponding receiver plate with ninety-six (96) individual wells that have a maximum 200 ? L working volume. Biofilm is established on the pegs under batch conditions (that is, no flow of nutrients into or out of an individual well) with gentle mixing. The established biofilm is transferred to a new receiver plate for disinfectant efficacy testing. The reactor design allows for the simultaneous testing of multiple disinfectants or one disinfectant with multiple concentrations, and replicate samples, making the assay an efficient screening tool.

1.2 This test method defines the specific operational parameters necessary for growing a Pseudomonas aeruginosa biofilm, although the device is versatile and has been used for growing, evaluating and/or studying biofilms of different species as seen in Refs (1-4) .

1.3 Validation of disinfectant neutralization is included as part of the assay.

1.4 This test method describes how to sample the biofilm and quantify viable cells. Biofilm population density is recorded as log colony forming units per surface area. Efficacy is reported as the log reduction of viable cells.

1.5 Basic microbiology training is required to perform this assay.

1.6 The values stated in SI units are to be regarded as standard. No other units of measurement are included in this standard.

1.7 ASTM International takes no position respecting the validity of any patent rights asserted in connection with any item mentioned in this standard. Users of this standard are expressly advised that determination of the validity of any such patent rights, and the risk of infringement of such rights, are entirely their own responsibility.

1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety and health practices and determine the applicability of regulatory limitations prior to use.


2. Referenced Documents (purchase separately) The documents listed below are referenced within the subject standard but are not provided as part of the standard.

ASTM Standards

E1054 Test Methods for Evaluation of Inactivators of Antimicrobial Agents

Other Standards

Method9050C.1.a Buffered Dilution Water Preparation according to Eaton et al ()

Keywords

biofilm; efficacy testing; growth reactor; MBEC; Pseudomonas aeruginosa ; sampling; Biofilm; Disinfectants/disinfection; Efficacy; Sampling


ICS Code

ICS Number Code 07.100.01 (Microbiology in general)


DOI: 10.1520/E2799-11

ASTM International is a member of CrossRef.


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ASTM International, formerly known as the American Society for Testing and Materials (ASTM), is a globally recognized leader in the development and delivery of international voluntary consensus standards. Today, some 12,000 ASTM standards are used around the world to improve product quality, enhance safety, facilitate market access and trade, and build consumer confidence. ASTM’s leadership in international standards development is driven by the contributions of its members: more than 30,000 of the world’s top technical experts and business professionals representing 150 countries. Working in an open and transparent process and using ASTM’s advanced electronic infrastructure, ASTM members deliver the test methods, specifications, guides, and practices that support industries and governments worldwide.

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